Explant-dependent receptivity to isolation and a cell-wall resynthesis in protoplast culture of recalcitrant yellow lupin
Cell-wall resynthesis was studied in protoplast culture of yellow lupin (Lupinus luteus L.). We optimized protoplast isolation and found that explants excised from young seedling were more suitable sources of protoplasts, in contrast to callus tissue. Incubation in 2% cellulase R-10, 1% pectinase an...
| Main Authors: | , |
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| Format: | Article |
| Language: | Bulgarian |
| Published: |
University of Zagreb, Faculty of Agriculture
2013-03-01
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| Series: | Journal of Central European Agriculture |
| Subjects: | |
| Online Access: | http://jcea.agr.hr/articles/774634_Explant_dependent_receptivity_to_isolation_and_a_cell_wall_resynthesis_in_protoplast_culture_of_recalcitrant_yellow_lupin_en.pdf |