A BaSiC tool for background and shading correction of optical microscopy images
Accurate quantification of bioimaging data is often confounded by uneven illumination (shading) in space and background variation in time. Here the authors present BaSiC, a Fiji plugin solving both issues. It requires fewer input images and is more robust to artefacts than existing shading correctio...
Main Authors: | , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2017-06-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/ncomms14836 |