Pairwise efficiency: a new mathematical approach to qPCR data analysis increases the precision of the calibration curve assay

Pairwise efficiency: a new mathematical approach to qPCR data analysis increases the precision of the calibration curve assay

Abstract Background The real-time quantitative polymerase chain reaction (qPCR) is routinely used for quantification of nucleic acids and is considered the gold standard in the field of relative nucleic acid measurements. The efficiency of the qPCR reaction is one of the most important parameters in...

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Bibliographic Details
Main Authors: Yulia Panina, Arno Germond, Brit G. David, Tomonobu M. Watanabe
Format: Article
Language:English
Published: BMC 2019-05-01
Series:BMC Bioinformatics
Subjects:
Quantitative PCR
Efficiency determination
Combinatorial treatment
Online Access:http://link.springer.com/article/10.1186/s12859-019-2911-5

Internet

http://link.springer.com/article/10.1186/s12859-019-2911-5

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