Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform

Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform

Optimizing an enzyme usually requires testing thousands of variants, thus consuming large amounts of material and time. Here, the authors present a method that allows for measuring two different activities of the same enzyme simultaneously instead of doing two consecutive rounds of screening.

Bibliographic Details
Main Authors: Fuqiang Ma, Meng Ting Chung, Yuan Yao, Robert Nidetz, Lap Man Lee, Allen P. Liu, Yan Feng, Katsuo Kurabayashi, Guang-Yu Yang
Format: Article
Language:English
Published: Nature Publishing Group 2018-03-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-018-03492-6
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spelling doaj-9f4f01f54d654f369fd23c54de3aefef2021-05-11T10:06:09ZengNature Publishing GroupNature Communications2041-17232018-03-01911810.1038/s41467-018-03492-6Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platformFuqiang Ma0Meng Ting Chung1Yuan Yao2Robert Nidetz3Lap Man Lee4Allen P. Liu5Yan Feng6Katsuo Kurabayashi7Guang-Yu Yang8State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong UniversityDepartment of Mechanical Engineering, University of MichiganMIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Harbin Institute of TechnologyDepartment of Mechanical Engineering, University of MichiganDepartment of Mechanical Engineering, University of MichiganDepartment of Mechanical Engineering, University of MichiganState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong UniversityDepartment of Mechanical Engineering, University of MichiganState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong UniversityOptimizing an enzyme usually requires testing thousands of variants, thus consuming large amounts of material and time. Here, the authors present a method that allows for measuring two different activities of the same enzyme simultaneously instead of doing two consecutive rounds of screening.https://doi.org/10.1038/s41467-018-03492-6
collection DOAJ
language English
format Article
sources DOAJ
author Fuqiang Ma
Meng Ting Chung
Yuan Yao
Robert Nidetz
Lap Man Lee
Allen P. Liu
Yan Feng
Katsuo Kurabayashi
Guang-Yu Yang
spellingShingle Fuqiang Ma
Meng Ting Chung
Yuan Yao
Robert Nidetz
Lap Man Lee
Allen P. Liu
Yan Feng
Katsuo Kurabayashi
Guang-Yu Yang
Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform
Nature Communications
author_facet Fuqiang Ma
Meng Ting Chung
Yuan Yao
Robert Nidetz
Lap Man Lee
Allen P. Liu
Yan Feng
Katsuo Kurabayashi
Guang-Yu Yang
author_sort Fuqiang Ma
title Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform
title_short Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform
title_full Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform
title_fullStr Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform
title_full_unstemmed Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform
title_sort efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2018-03-01
description Optimizing an enzyme usually requires testing thousands of variants, thus consuming large amounts of material and time. Here, the authors present a method that allows for measuring two different activities of the same enzyme simultaneously instead of doing two consecutive rounds of screening.
url https://doi.org/10.1038/s41467-018-03492-6
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