Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform
Optimizing an enzyme usually requires testing thousands of variants, thus consuming large amounts of material and time. Here, the authors present a method that allows for measuring two different activities of the same enzyme simultaneously instead of doing two consecutive rounds of screening.
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2018-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-018-03492-6 |