Replacement of serum supplemented medium for CB.Hep-1 hybridoma cell freezing and monoclonal antibody production

• Main Authors: Hasel Aragón, Marcos González, Rodolfo Valdés, Tatiana Álvarez, Enma Brown, Yadira Rodríguez, Tatiana González, Regla Somoza, Mayra Wood
• Format: Article
• Language: English
• Published: Elfos Scientiae
• Series: Biotecnología Aplicada
• Subjects: cell freezing medium; monoclonal antibody; protein free medium; serum-free medium
• Online Access: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1027-28522013000100007&lng=en&tlng=en

CB.Hep-1 monoclonal antibody (mAb) has been used in Hepatitis B vaccine manufacture. The mAb production was performed by ascites method inoculating cells previously frozen and cultivated in fetal bovine serum supplemented medium (SSM). It is expensive at risk and can be contaminated with adventitious pathogens. Therefore, the main objective of this study was to replace the SSM by the chemically defined medium Filocethplus (cell freezing) and the protein free medium HyQ(r)SFM4CHOTM (cell growing). As results, cells cryo-preserved in Filocethplus for 180 days and cultivated in HyQ(r)SFM4CHOTM showed no significant differences in regards to viability, maximum viable cell density and growth conditions with the same cells cultivated and frozen in SSM. In addition, CB.Hep-1 cells that were frozen in Filocethplus and cultivated in HyQ(r)SFM4CHOTM exhibited similar performance to those frozen and cultivated in SSM in agitated culture, which point out the application of these media for CB.Hep-1 mAb and Hepatitis B vaccine production.