Replacement of serum supplemented medium for CB.Hep-1 hybridoma cell freezing and monoclonal antibody production
CB.Hep-1 monoclonal antibody (mAb) has been used in Hepatitis B vaccine manufacture. The mAb production was performed by ascites method inoculating cells previously frozen and cultivated in fetal bovine serum supplemented medium (SSM). It is expensive at risk and can be contaminated with adventitiou...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elfos Scientiae
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| Series: | Biotecnología Aplicada |
| Subjects: | |
| Online Access: | http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1027-28522013000100007&lng=en&tlng=en |