Enhancing the Heterologous Fructosyltransferase Activity of Kluyveromyces lactis: Developing a Scaled-Up Process and Abolishing Invertase by CRISPR/Cas9 Genome Editing

Enhancing the Heterologous Fructosyltransferase Activity of Kluyveromyces lactis: Developing a Scaled-Up Process and Abolishing Invertase by CRISPR/Cas9 Genome Editing

The enzymatic production of prebiotic fructo-oligosaccharides (FOS) from sucrose involves fructosyltransferases (FFTs) and invertases, both of which catalyze forward (transferase) and reverse (hydrolysis) reactions. FOS yields can therefore be increased by favoring the forward reaction. We investiga...

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Bibliographic Details
Main Authors: Jan Philipp Burghardt, Rong Fan, Markus Baas, Dustin Eckhardt, Doreen Gerlach, Peter Czermak
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-11-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
chemically-defined fermentation medium
CRISPR/Cas9
fructosyltransferase (FFT)
prebiotic
Kluyveromyces lactis GG799
fructo-oligosaccharides (FOS)
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2020.607507/full

Internet

https://www.frontiersin.org/articles/10.3389/fbioe.2020.607507/full

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