Genome Editing in Zebrafish by ScCas9 Recognizing NNG PAM
The CRISPR/Cas9 system has been widely used for gene editing in zebrafish. However, the required NGG protospacer adjacent motif (PAM) of <i>Streptococcus pyogenes</i> Cas9 (SpCas9) notably restricts the editable range of the zebrafish genome. Recently, Cas9 from <i>S. canis</i&g...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-08-01
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| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/10/8/2099 |