Comparison of direct immunofluorescence, conventional cell culture and polymerase chain reaction techniques for detecting respiratory syncytial virus in nasopharyngeal aspirates from infants Comparação entre imunofluorescência direta, cultura convencional de células e reação em cadeia de polimerase para detectar vírus respiratório sincicial em lavados de nasofaringe de crianças

Comparison of direct immunofluorescence, conventional cell culture and polymerase chain reaction techniques for detecting respiratory syncytial virus in nasopharyngeal aspirates from infants Comparação entre imunofluorescência direta, cultura convencional de células e reação em cadeia de polimerase para detectar vírus respiratório sincicial em lavados de nasofaringe de crianças

A total of 316 samples of nasopharyngeal aspirate from infants up to two years of age with acute respiratory-tract illnesses were processed for detection of respiratory syncytial virus (RSV) using three different techniques: viral isolation, direct immunofluorescence, and PCR. Of the samples, 36 (11...

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Bibliographic Details
Main Authors: Alexanda Dias Reis, Maria Cristina Domingues Fink, Clarisse Martins Machado, José de Paula Paz Jr., Renato Reis Oliveira, Adriana Fumie Tateno, Adriana Freire Machado, Maria Regina Cardoso, Claudio Sérgio Pannuti
Format: Article
Language:English
Published: Universidade de São Paulo 2008-02-01
Series:Revista do Instituto de Medicina Tropical de São Paulo
Subjects:
Respiratory syncytial virus
Infants
Diagnosis
Cell culture
Polymerase chain reaction
Immunofluorescence
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652008000100008

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652008000100008

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