Zymographic differentiation of [NiFe]-Hydrogenases 1, 2 and 3 of <it>Escherichia coli</it> K-12

Zymographic differentiation of [NiFe]-Hydrogenases 1, 2 and 3 of <it>Escherichia coli</it> K-12

<p>Abstract</p> <p>Background</p> <p>When grown under anaerobic conditions, <it>Escherichia coli</it> K-12 is able to synthesize three active [NiFe]-hydrogenases (Hyd1-3). Two of these hydrogenases are respiratory enzymes catalysing hydrogen oxidation, where...

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Bibliographic Details
Main Authors: Pinske Constanze, Jaroschinsky Monique, Sargent Frank, Sawers Gary
Format: Article
Language:English
Published: BMC 2012-07-01
Series:BMC Microbiology
Subjects:
NiFe
Hydrogenase
Formate hydrogenlyase
Formate dehydrogenase
Non-denaturating polyacrylamide gel electrophoresis
In-gel activity staining
Redox-dyes
Online Access:http://www.biomedcentral.com/1471-2180/12/134

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http://www.biomedcentral.com/1471-2180/12/134

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