In vivo determination of organellar pH using a universal wavelength-based confocal microscopy approach.

Many essential cellular processes are affected by transmembrane H(+) gradients and intracellular pH (pHi). The research of such metabolic events calls for a non-invasive method to monitor pHi within individual subcellular compartments. We present a novel confocal microscopy approach for the determin...

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Bibliographic Details
Main Authors: Albert Pineda Rodó, Libuše Váchová, Zdena Palková
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3310042?pdf=render