Isolation and identification of new constituents in milk fat

After removal of cholesterol on a digitonin column, the unsaponifiable matter of milk fat was examined for alcoholic substituents. Derivatization with pyruvic acid chloride 2,6-dinitrophenylhydrazone and fractionation of the derivatives gave four main fractions. The second, the hexane-benzene fracti...

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Main Authors:	C.R. Brewington, E.A. Caress, D.P. Schwartz
Format:	Article
Language:	English
Published:	Elsevier 1970-07-01
Series:	Journal of Lipid Research
Subjects:	unsaponifiable matter digitonin column gas-liquid chromatography mass spectrometry lanosterol dihydrolanosterol
Online Access:	http://www.sciencedirect.com/science/article/pii/S0022227520429724

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spelling	doaj-9acc8596db4e4864b33df3c71d5194292021-04-24T05:54:53ZengElsevierJournal of Lipid Research0022-22751970-07-01114355361Isolation and identification of new constituents in milk fatC.R. Brewington0E.A. Caress1D.P. Schwartz2Dairy Products Laboratory, Eastern Utilization Research and Development Division, Agricultural Research Service, U.S. Department of Agriculture, Washington, D.C. 20250Dairy Products Laboratory, Eastern Utilization Research and Development Division, Agricultural Research Service, U.S. Department of Agriculture, Washington, D.C. 20250Dairy Products Laboratory, Eastern Utilization Research and Development Division, Agricultural Research Service, U.S. Department of Agriculture, Washington, D.C. 20250After removal of cholesterol on a digitonin column, the unsaponifiable matter of milk fat was examined for alcoholic substituents. Derivatization with pyruvic acid chloride 2,6-dinitrophenylhydrazone and fractionation of the derivatives gave four main fractions. The second, the hexane-benzene fraction, was shown by thin-layer chromatography to have a mobility similar to many common sterols. The hexane-benzene fraction was saponified and gave rise to free alcohols, which were then analyzed on a combination gas-liquid chromatograph-mass spectrometer. Dihydrolanosterol, previously unreported in milk fat, and lanosterol, previously identified but never confirmed, were characterized. The sterols which were precipitated on the digitonin column, were removed, and by the use of the combination gas-liquid chromatograph-mass spectrometer β-sitosterol was identified. In addition, lanosterol and dihydrolanosterol were isolated from the unsaponifiable matter by chromatography on Florisil.http://www.sciencedirect.com/science/article/pii/S0022227520429724unsaponifiable matterdigitonin columngas-liquid chromatographymass spectrometrylanosteroldihydrolanosterol
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	C.R. Brewington E.A. Caress D.P. Schwartz
spellingShingle	C.R. Brewington E.A. Caress D.P. Schwartz Isolation and identification of new constituents in milk fat Journal of Lipid Research unsaponifiable matter digitonin column gas-liquid chromatography mass spectrometry lanosterol dihydrolanosterol
author_facet	C.R. Brewington E.A. Caress D.P. Schwartz
author_sort	C.R. Brewington
title	Isolation and identification of new constituents in milk fat
title_short	Isolation and identification of new constituents in milk fat
title_full	Isolation and identification of new constituents in milk fat
title_fullStr	Isolation and identification of new constituents in milk fat
title_full_unstemmed	Isolation and identification of new constituents in milk fat
title_sort	isolation and identification of new constituents in milk fat
publisher	Elsevier
series	Journal of Lipid Research
issn	0022-2275
publishDate	1970-07-01
description	After removal of cholesterol on a digitonin column, the unsaponifiable matter of milk fat was examined for alcoholic substituents. Derivatization with pyruvic acid chloride 2,6-dinitrophenylhydrazone and fractionation of the derivatives gave four main fractions. The second, the hexane-benzene fraction, was shown by thin-layer chromatography to have a mobility similar to many common sterols. The hexane-benzene fraction was saponified and gave rise to free alcohols, which were then analyzed on a combination gas-liquid chromatograph-mass spectrometer. Dihydrolanosterol, previously unreported in milk fat, and lanosterol, previously identified but never confirmed, were characterized. The sterols which were precipitated on the digitonin column, were removed, and by the use of the combination gas-liquid chromatograph-mass spectrometer β-sitosterol was identified. In addition, lanosterol and dihydrolanosterol were isolated from the unsaponifiable matter by chromatography on Florisil.
topic	unsaponifiable matter digitonin column gas-liquid chromatography mass spectrometry lanosterol dihydrolanosterol
url	http://www.sciencedirect.com/science/article/pii/S0022227520429724
work_keys_str_mv	AT crbrewington isolationandidentificationofnewconstituentsinmilkfat AT eacaress isolationandidentificationofnewconstituentsinmilkfat AT dpschwartz isolationandidentificationofnewconstituentsinmilkfat
_version_	1721511342189314048

Isolation and identification of new constituents in milk fat

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