Cloning, characterization of two female-specific AFLP markers and development of PCR-based sex identification method for the half-smooth tongue sole Cynoglossus semilaevis

Cloning, characterization of two female-specific AFLP markers and development of PCR-based sex identification method for the half-smooth tongue sole Cynoglossus semilaevis

Two female-specific AFLP (amplified fragment length polymorphism) markers (named CseF464 and CseF136) were isolated by using one selective primer combination (E-AGC/M-CTG) from the genomic DNA of 20 females and 20 males of the half-smooth tongue sole Cynoglossus semilaevis. Both the markers were re-...

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Bibliographic Details
Main Author: Hongyu MA, Songlin CHEN, Jing LI, Jin-Zhen BI, Tianjun XU
Format: Article
Language:English
Published: Oxford University Press 2009-08-01
Series:Current Zoology
Subjects:
AFLP
Cynoglossus semilaevis
Female-specific marker
SCAR
Molecular sexing
Online Access:http://www.currentzoology.org/paperdetail.asp?id=11230

Internet

http://www.currentzoology.org/paperdetail.asp?id=11230

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