2-(2-Hydroxy-5-nitrobenzylidene)-1,3-indanedione versus Fluorescein Isothiocyanate in Interaction with Anti-hFABP Immunoglobulin G1: Fluorescence Quenching, Secondary Structure Alteration and Binding Sites Localization

2-(2-Hydroxy-5-nitrobenzylidene)-1,3-indanedione versus Fluorescein Isothiocyanate in Interaction with Anti-hFABP Immunoglobulin G1: Fluorescence Quenching, Secondary Structure Alteration and Binding Sites Localization

The first step in determining whether a fluorescent dye can be used for antibody labeling consists in collecting data on its physical interaction with the latter. In the present study, the interaction between the 2-(2-hydroxy-5-nitrobenzylidene)-1,3-indanedione (HNBID) dye and the IgG1 monoclonal mo...

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Bibliographic Details
Main Authors: Iulia Matei, Mihaela Savin, Dana Stan, Carmen-Marinela Mihailescu
Format: Article
Language:English
Published: MDPI AG 2013-01-01
Series:International Journal of Molecular Sciences
Subjects:
indane-1,3-dione derivative
fluorescein isothiocyanate
immunoglobulin G1
fluorescence spectroscopy
circular dichroism
molecular modeling
Online Access:http://www.mdpi.com/1422-0067/14/2/3011

Internet

http://www.mdpi.com/1422-0067/14/2/3011

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