Tailored co-localization analysis of intracellular microbes and punctum-distributed phagosome–lysosome pathway proteins using ImageJ plugin EzColocalization

Abstract Immunofluorescence is indispensable to monitor redistribution of proteins involved in phagosome–lysosome association pathway-relevant (P–LApr) proteins. The software digitizing the signals of these proteins in an unbiased and automated manner is generally costly and not widely available. Th...

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Bibliographic Details
Main Authors: Kang Wu, Bo Yan, Douglas B. Lowrie, Tao Li, Xiao-Yong Fan
Format: Article
Language:English
Published: Nature Publishing Group 2021-01-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-020-79425-5