Using single loxP sites to enhance homologous recombination: ts mutants in Sec1 of Dictyostelium discoideum.

Dictyostelium discoideum amoebae are haploid and, as they share many features with animal cells, should be an ideal creature for studying basic processes such as cell locomotion. Isolation of mutants in this amoeba has largely been limited to non-essential genes: nsfA-the gene for NEM-sensitive fact...

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Bibliographic Details
Main Authors: Mark S Bretscher, Margaret Clotworthy
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2007-08-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC1933600?pdf=render