Metagenomic screening for aromatic compound-responsive transcriptional regulators.

We applied a metagenomics approach to screen for transcriptional regulators that sense aromatic compounds. The library was constructed by cloning environmental DNA fragments into a promoter-less vector containing green fluorescence protein. Fluorescence-based screening was then performed in the pres...

Full description

Bibliographic Details
Main Authors: Taku Uchiyama, Kentaro Miyazaki
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3786939?pdf=render