Development of an in vitro compartmentalization screen for high-throughput directed evolution of [FeFe] hydrogenases.

BACKGROUND: [FeFe] hydrogenase enzymes catalyze the formation and dissociation of molecular hydrogen with the help of a complex prosthetic group composed of common elements. The development of energy conversion technologies based on these renewable catalysts has been hindered by their extreme oxygen...

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Bibliographic Details
Main Authors: James A Stapleton, James R Swartz
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2010-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2997796?pdf=render