A rapid biosynthetic method for the preparation of radioactive phosphatidyl-CMP(CDP-diacylglycerol) of high specific activity.
A procedrue is described for the preparation of [32P]phosphatidyl-CMP(CDP-diacylglycerol) from rat pineal glands incubated with [32P]orthophosphate and DL-propranolol. The product is 95% radiopure and a high specific acitivity. The yield of liponucleotide is 0.4–0.9 micron Ci/mCi of [32P]orthophosph...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1978-08-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520412817 |