An Optimized microRNA Backbone for Effective Single-Copy RNAi

An Optimized microRNA Backbone for Effective Single-Copy RNAi

Short hairpin RNA (shRNA) technology enables stable and regulated gene repression. For establishing experimentally versatile RNAi tools and minimizing toxicities, synthetic shRNAs can be embedded into endogenous microRNA contexts. However, due to our incomplete understanding of microRNA biogenesis,...

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Bibliographic Details
Main Authors: Christof Fellmann, Thomas Hoffmann, Vaishali Sridhar, Barbara Hopfgartner, Matthias Muhar, Mareike Roth, Dan Yu Lai, Inês A.M. Barbosa, Jung Shick Kwon, Yuanzhe Guan, Nishi Sinha, Johannes Zuber
Format: Article
Language:English
Published: Elsevier 2013-12-01
Series:Cell Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124713006876

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http://www.sciencedirect.com/science/article/pii/S2211124713006876

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