Expression, production, and renaturation of a functional single-chain variable antibody fragment (scFv) against human ICAM-1

Expression, production, and renaturation of a functional single-chain variable antibody fragment (scFv) against human ICAM-1

Intercellular adhesion molecule-1 (ICAM-1) is an important factor in the progression of inflammatory responses in vivo. To develop a new anti-inflammatory drug to block the biological activity of ICAM-1, we produced a monoclonal antibody (Ka=4.19×10−8 M) against human ICAM-1. The anti-ICAM-1 single-...

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Bibliographic Details
Main Authors: H. Sun, G.M. Wu, Y.Y. Chen, Y. Tian, Y.H. Yue, G.L. Zhang
Format: Article
Language:English
Published: Associação Brasileira de Divulgação Científica 2014-07-01
Series:Brazilian Journal of Medical and Biological Research
Subjects:
Intercellular adhesion molecule-1
Single-chain variable antibody fragment
Expression
Purification
Renaturation
Biological activity
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700540&lng=en&tlng=en

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700540&lng=en&tlng=en

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