Reverse-genetics studies of lncRNAs—what we have learnt and paths forward

Reverse-genetics studies of lncRNAs—what we have learnt and paths forward

Abstract Long non-coding RNAs (lncRNAs) represent a major fraction of the transcriptome in multicellular organisms. Although a handful of well-studied lncRNAs are broadly recognized as biologically meaningful, the fraction of such transcripts out of the entire collection of lncRNAs remains a subject...

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Main Authors: Fan Gao, Ye Cai, Philipp Kapranov, Dongyang Xu
Format: Article
Language:English
Published: BMC 2020-04-01
Series:Genome Biology
Online Access:http://link.springer.com/article/10.1186/s13059-020-01994-5
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spelling doaj-979ebc59b14a4632a36d557960514cf62020-11-25T02:28:22ZengBMCGenome Biology1474-760X2020-04-0121112310.1186/s13059-020-01994-5Reverse-genetics studies of lncRNAs—what we have learnt and paths forwardFan Gao0Ye Cai1Philipp Kapranov2Dongyang Xu3Institute of Genomics, School of Biomedical Sciences, Huaqiao UniversityInstitute of Genomics, School of Biomedical Sciences, Huaqiao UniversityInstitute of Genomics, School of Biomedical Sciences, Huaqiao UniversityInstitute of Genomics, School of Biomedical Sciences, Huaqiao UniversityAbstract Long non-coding RNAs (lncRNAs) represent a major fraction of the transcriptome in multicellular organisms. Although a handful of well-studied lncRNAs are broadly recognized as biologically meaningful, the fraction of such transcripts out of the entire collection of lncRNAs remains a subject of vigorous debate. Here we review the evidence for and against biological functionalities of lncRNAs and attempt to arrive at potential modes of lncRNA functionality that would reconcile the contradictory conclusions. Finally, we discuss different strategies of phenotypic analyses that could be used to investigate such modes of lncRNA functionality.http://link.springer.com/article/10.1186/s13059-020-01994-5
collection DOAJ
language English
format Article
sources DOAJ
author Fan Gao
Ye Cai
Philipp Kapranov
Dongyang Xu
spellingShingle Fan Gao
Ye Cai
Philipp Kapranov
Dongyang Xu
Reverse-genetics studies of lncRNAs—what we have learnt and paths forward
Genome Biology
author_facet Fan Gao
Ye Cai
Philipp Kapranov
Dongyang Xu
author_sort Fan Gao
title Reverse-genetics studies of lncRNAs—what we have learnt and paths forward
title_short Reverse-genetics studies of lncRNAs—what we have learnt and paths forward
title_full Reverse-genetics studies of lncRNAs—what we have learnt and paths forward
title_fullStr Reverse-genetics studies of lncRNAs—what we have learnt and paths forward
title_full_unstemmed Reverse-genetics studies of lncRNAs—what we have learnt and paths forward
title_sort reverse-genetics studies of lncrnas—what we have learnt and paths forward
publisher BMC
series Genome Biology
issn 1474-760X
publishDate 2020-04-01
description Abstract Long non-coding RNAs (lncRNAs) represent a major fraction of the transcriptome in multicellular organisms. Although a handful of well-studied lncRNAs are broadly recognized as biologically meaningful, the fraction of such transcripts out of the entire collection of lncRNAs remains a subject of vigorous debate. Here we review the evidence for and against biological functionalities of lncRNAs and attempt to arrive at potential modes of lncRNA functionality that would reconcile the contradictory conclusions. Finally, we discuss different strategies of phenotypic analyses that could be used to investigate such modes of lncRNA functionality.
url http://link.springer.com/article/10.1186/s13059-020-01994-5
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AT yecai reversegeneticsstudiesoflncrnaswhatwehavelearntandpathsforward
AT philippkapranov reversegeneticsstudiesoflncrnaswhatwehavelearntandpathsforward
AT dongyangxu reversegeneticsstudiesoflncrnaswhatwehavelearntandpathsforward
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