Simultaneous Screening of 24 Target Genes of Foodborne Pathogens in 35 Foodborne Outbreaks Using Multiplex Real-Time SYBR Green PCR Analysis

A set of 8 multiplex real-time SYBR Green PCR (SG-PCR) assays including 3 target primers and an internal amplification control (IAC) primer was simultaneously evaluated in 3 h or less with regard to detection of 24 target genes of 23 foodborne pathogens in 7 stool specimens of foodborne outbreak usi...

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Bibliographic Details
Main Authors: Hiroshi Fukushima, Jun Kawase, Yoshiki Etoh, Kumiko Sugama, Shunshuke Yashiro, Natsuko Iida, Keiji Yamaguchi
Format: Article
Language:English
Published: Hindawi Limited 2010-01-01
Series:International Journal of Microbiology
Online Access:http://dx.doi.org/10.1155/2010/864817