Quick and easy purification of murine untouched naive B cells or germinal center B cells by MACS

Summary: Humoral immune responses depend on the generation of high-affinity antigen-specific antibodies. Germinal center (GC) B cells are the cornerstone of this response in peripheral lymphoid organs. High purities of GC B cells, and also naive B cells, are required for accurate analysis in downstr...

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Bibliographic Details
Main Authors: Parham Ramezani-Rad, Robert C. Rickert
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721000769