Extraction of HCV-RNA from Plasma Samples: Development towards Semiautomation

A semiautomated extraction protocol of HCV-RNA using Favorgen RNA extraction kit has been developed. The kit provided protocol was modified by replacing manual spin steps with vacuum filtration. The assay performance was evaluated by real-time qPCR based on Taqman technology. Assay linearity was con...

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Bibliographic Details
Main Authors: Imran Amin, Tania Jabbar, Fawad Niazi, Muhammad Saeed Akhtar
Format: Article
Language:English
Published: Hindawi Limited 2015-01-01
Series:International Journal of Analytical Chemistry
Online Access:http://dx.doi.org/10.1155/2015/367801