Extraction of HCV-RNA from Plasma Samples: Development towards Semiautomation
A semiautomated extraction protocol of HCV-RNA using Favorgen RNA extraction kit has been developed. The kit provided protocol was modified by replacing manual spin steps with vacuum filtration. The assay performance was evaluated by real-time qPCR based on Taqman technology. Assay linearity was con...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2015-01-01
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Series: | International Journal of Analytical Chemistry |
Online Access: | http://dx.doi.org/10.1155/2015/367801 |