Optimization of heterologous expression of banana glucanase in E. coli

Optimization of heterologous expression of banana glucanase in E. coli

For the heterologous production of a banana glucanase in Escherichia coli, its gene (GenBank GQ268963) was cloned into a pGEX-4T expression vector as a fusion protein with glutathione-S-transferase (GST). BL21 cells transformed with the GST-Mus a 5 construct were employed for the protein producti...

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Bibliographic Details
Main Authors: Abughren Mohamed, Popović Milica, Dimitrijević Rajna, Burazer Lidija, Grozdanović Milica, Atanasković-Marković Marina, Gavrović-Jankulović Marija
Format: Article
Language:English
Published: Serbian Chemical Society 2012-01-01
Series:Journal of the Serbian Chemical Society
Subjects:
food allergen
protein expression
glucanase
Online Access:http://www.doiserbia.nb.rs/img/doi/0352-5139/2012/0352-51391100158A.pdf

Internet

http://www.doiserbia.nb.rs/img/doi/0352-5139/2012/0352-51391100158A.pdf

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