TROUBLESHOOTING IN EXPRESSION AND PURIFICATION OF RECOMBINANT SEVERE ACUTE RESPIRATORY SYNDROME-ASSOCIATED CORONAVIRUS NUCLEOCAPSID PROTEIN IN Escherichia coli BL21
Considering importance of N protein for study of viral pathogenesis or development of immunodiagnostic assay, wereported effects of several conditions on purity and homogeneity of recombinant SARS-CoV N protein expressed in E.coli BL21. The SARS-CoV N gene was reverse transcribed and amplified by th...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Universitas Indonesia
2010-11-01
|
Series: | Makara Seri Sains |
Subjects: | |
Online Access: | http://journal.ui.ac.id/science/article/viewFile/741/704 |