Microcarrier-based expansion of adult murine side population stem cells.

The lack of reliable methods to efficiently isolate and propagate stem cell populations is a significant obstacle to the advancement of cell-based therapies for human diseases. One isolation technique is based on efflux of the fluorophore Hoechst 33342. Using fluorescence-activated cell sorting (FAC...

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Bibliographic Details
Main Authors: Christina A Pacak, Mau-Thek Eddy, Lindsey Woodhull, Kai-Roy Wang, Ivan Alpatov, Shelby Fullen, Rory P Dowd, Yeong-Hoon Choi, Douglas B Cowan
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3557248?pdf=render