The selective biotin tagging and thermolysin proteolysis of chloroplast outer envelope proteins reveals information on protein topology and association into complexes

The selective biotin tagging and thermolysin proteolysis of chloroplast outer envelope proteins reveals information on protein topology and association into complexes

The understanding of chloroplast function requires the precise localization of proteins in each of its sub-compartments. High-sensitivity mass spectrometry has allowed the inventory of proteins in thylakoid, stroma and envelope fractions. Concerning membrane association, proteins can be either integ...

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Bibliographic Details
Main Authors: Hélène eHardré, Lauriane eKuhn, Catherine eAlbrieux, Juliette eJouhet, Morgane eMichaud, Daphné eSeigneurin-Berny, Denis eFalconet, Maryse A. Block, Eric eMarechal
Format: Article
Language:English
Published: Frontiers Media S.A. 2014-05-01
Series:Frontiers in Plant Science
Subjects:
Biotinylation
Chloroplast envelope
outer envelope protein
membrane protein complexes
thermolysin.
Online Access:http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00203/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00203/full

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