Successful Transfer of a Model T-DNA Plasmid to E. coli Revealed Its Dependence on Recipient RecA and the Preference of VirD2 Relaxase for Eukaryotes Rather Than Bacteria as Recipients

Successful Transfer of a Model T-DNA Plasmid to E. coli Revealed Its Dependence on Recipient RecA and the Preference of VirD2 Relaxase for Eukaryotes Rather Than Bacteria as Recipients

In Agrobacterium-mediated transformation (AMT) of plants, a single-strand (ss) T-DNA covalently linked with a VirD2 protein moves through a bacterial type IV secretion channel called VirB/D4. This transport system originates from conjugal plasmid transfer systems of bacteria. The relaxase VirD2 and...

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Bibliographic Details
Main Authors: Yuta Ohmine, Kazuya Kiyokawa, Kazuya Yunoki, Shinji Yamamoto, Kazuki Moriguchi, Katsunori Suzuki
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-05-01
Series:Frontiers in Microbiology
Subjects:
horizontal DNA transfer
conjugation
recA
T-DNA transfer
type 4 secretion system
Agrobacterium
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2018.00895/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2018.00895/full

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