Development and Application of a High Throughput Protein Unfolding Kinetic Assay.

The kinetics of folding and unfolding underlie protein stability and quantification of these rates provides important insights into the folding process. Here, we present a simple high throughput protein unfolding kinetic assay using a plate reader that is applicable to the studies of the majority of...

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Bibliographic Details
Main Authors: Qiang Wang, Nicklas Waterhouse, Olusegun Feyijinmi, Matthew J Dominguez, Lisa M Martinez, Zoey Sharp, Rachel Service, Jameson R Bothe, Elliott J Stollar
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4706425?pdf=render