A simple procedure for rehybridization of nuclei analyzed previously by fish

We present a simple procedure for interphase fluorescence in situ rehybridization (FISH). This procedure was used to evaluate fresh prostate tumor from needle biopsy specimens. Digoxigenin-labeled DNA probes were hybridized onto nuclei which had been previously investigated by FISH using biotin-labe...

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Bibliographic Details
Main Authors: Andréa Borduchi Carvalho-Salles, Nilce Barril, Eloiza Helena Tajara
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 1999-06-01
Series:Genetics and Molecular Biology
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200006