Retrieval and purification of human β cells from stem cell-derived islet engrafted mice

Summary: Stem cell-derived beta cells (SC-β-cells) engrafted into mice serve as a pre-clinical model of diabetes. It is helpful to recover viable β cells following transplantation to perform tests on the graft. We developed a protocol to retrieve and purify a sufficient number of live β cells from m...

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Bibliographic Details
Main Authors: Jennifer Hyoje-Ryu Kenty, Gabriela Cristina Pelayo, Nayara Carvalho Leite
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721003828