Protocol for constructing a versatile tiling light sheet microscope for imaging cleared tissues

Summary: Here, we describe a protocol to construct, calibrate, and operate a versatile tiling light sheet microscope for imaging cleared tissues. The microscope uses adjustable tiling light sheets to achieve higher spatial resolution and better optical sectioning ability than conventional light shee...

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Bibliographic Details
Main Authors: Ruili Feng, Dongyue Wang, Yanlu Chen, Jing Lu, Liang Gao
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721002537