Saturated excitation microscopy using differential excitation for efficient detection of nonlinear fluorescence signals
We report a method to increase the efficiency of detecting nonlinear fluorescence signals in saturated excitation (SAX) microscopy. With this method, we compare fluorescence signals obtained under different degrees of saturated excitation to extract the nonlinear fluorescent signal induced by satura...
Main Authors: | , , , , , , |
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Format: | Article |
Language: | English |
Published: |
AIP Publishing LLC
2018-08-01
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Series: | APL Photonics |
Online Access: | http://dx.doi.org/10.1063/1.5039567 |