RAP Tag and PMab-2 Antibody: A Tagging System for Detecting and Purifying Proteins in Plant Cells

RAP Tag and PMab-2 Antibody: A Tagging System for Detecting and Purifying Proteins in Plant Cells

An affinity tag system requires both high affinity and specificity. The RAP tag epitope DMVNPGLEDRIE, derived from rat podoplanin (PDPN), is specifically recognized by PMab-2 monoclonal antibodies in rats. Here, we demonstrated that high levels of PMab-2 can be produced in Nicotiana benthamiana and...

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Bibliographic Details
Main Authors: Kenji Miura, Hideki Yoshida, Shohei Nosaki, Mika K. Kaneko, Yukinari Kato
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-09-01
Series:Frontiers in Plant Science
Subjects:
transient expression
tagging system
RAP tag
agroinfiltration
monoclonal antibody
protein expression
Online Access:https://www.frontiersin.org/article/10.3389/fpls.2020.510444/full

Internet

https://www.frontiersin.org/article/10.3389/fpls.2020.510444/full

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