pH-Dependent Deformations of the Energy Landscape of Avidin-like Proteins Investigated by Single Molecule Force Spectroscopy

pH-Dependent Deformations of the Energy Landscape of Avidin-like Proteins Investigated by Single Molecule Force Spectroscopy

Avidin and avidin-like proteins are widely used in numerous techniques since the avidin-biotin interaction is known to be very robust and reliable. Within this study, we investigated this bond at the molecular level under harsh conditions ranging from very low to very high pH values. We compared avi...

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Bibliographic Details
Main Authors: Melanie Köhler, Andreas Karner, Michael Leitner, Vesa P. Hytönen, Markku Kulomaa, Peter Hinterdorfer, Andreas Ebner
Format: Article
Language:English
Published: MDPI AG 2014-08-01
Series:Molecules
Subjects:
avidin mutant
avidin-biotin
force spectroscopy
molecular recognition
pH dependence
single molecules
biophysics
Online Access:http://www.mdpi.com/1420-3049/19/8/12531

Internet

http://www.mdpi.com/1420-3049/19/8/12531

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