A novel universal primer-multiplex-PCR method with sequencing gel electrophoresis analysis.

In this study, a novel universal primer-multiplex-PCR (UP-M-PCR) method adding a universal primer (UP) in the multiplex PCR reaction system was described. A universal adapter was designed in the 5'-end of each specific primer pairs which matched with the specific DNA sequences for each template...

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Bibliographic Details
Main Authors: Wentao Xu, Zhifang Zhai, Kunlun Huang, Nan Zhang, Yanfang Yuan, Ying Shang, Yunbo Luo
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3260127?pdf=render