Cytokine RT-qPCR and ddPCR for immunological investigations of the endangered Australian sea lion (Neophoca cinerea) and other mammals
Measurement of cytokine gene expression by reverse transcription quantitative polymerase chain reaction (RT-qPCR) is used widely to assess the immune system of animals and to identify biomarkers of disease, but its application is limited in wildlife species due to a lack of species-specific reagents...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
PeerJ Inc.
2020-11-01
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Series: | PeerJ |
Subjects: | |
Online Access: | https://peerj.com/articles/10306.pdf |