Web-based LinRegPCR: application for the visualization and analysis of (RT)-qPCR amplification and melting data

Abstract Background The analyses of amplification and melting curves have been shown to provide valuable information on the quality of the individual reactions in quantitative PCR (qPCR) experiments and to result in more reliable and reproducible quantitative results. Implementation The main steps i...

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Bibliographic Details
Main Authors: Andreas Untergasser, Jan M. Ruijter, Vladimir Benes, Maurice J. B. van den Hoff
Format: Article
Language:English
Published: BMC 2021-08-01
Series:BMC Bioinformatics
Subjects:
PCR
Online Access:https://doi.org/10.1186/s12859-021-04306-1