Histochemical detection of GM1 ganglioside using cholera toxin-B subunit. Evaluation of critical factors optimal for in situ detection with special emphasis to acetone pre-extraction

Histochemical detection of GM1 ganglioside using cholera toxin-B subunit. Evaluation of critical factors optimal for in situ detection with special emphasis to acetone pre-extraction

A comparison of histochemical detection of GM1 ganglioside in cryostat sections using cholera toxin B-subunit after fixation with 4% formaldehyde and dry acetone gave tissue-dependent results. In the liver no pre-treatment showed detectable differences related to GM1 reaction products, while studies...

Full description

Bibliographic Details
Main Authors: T. Petr, V. Šmíd, J. Šmídová, H. Hůlková, M. Jirkovská, M. Elleder, L. Muchová, L. Vitek, F. Šmíd
Format: Article
Language:English
Published: PAGEPress Publications 2010-05-01
Series:European Journal of Histochemistry
Subjects:
fixation, GM1 ganglioside, cholera toxin, anhydrous acetone, 4% formaldehyde
Online Access:http://www.ejh.it/index.php/ejh/article/view/1493

Internet

http://www.ejh.it/index.php/ejh/article/view/1493

Similar Items