Cloning and expression of phosphoenolpyruvate carboxykinase from Escherichia coli

In order to better study the structure and function of phosphoenolpyruvate carboxylase, the phosphoenolpyruvate carboxylase gene is cloned and expressed by PCR (polymerase chain reaction), double enzyme digestion and cell transformation. The results show that the new phosphoenolpyruvate carboxylase...

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Bibliographic Details
Main Authors: Xiaoliang HE, Zhu2 LI, Liqiang2 LIANG, Wanli MENG, Lantian MIAO, Yaozu SUN, Hongguang2 CAI
Format: Article
Language:zho
Published: Hebei University of Science and Technology 2019-04-01
Series:Journal of Hebei University of Science and Technology
Subjects:
CO2
Online Access:http://xuebao.hebust.edu.cn/hbkjdx/ch/reader/create_pdf.aspx?file_no=b201902006&flag=1&journal_