Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification
The purpose of this work was to study a rapid yeast DNA extraction by boiling and freeze-thawing processes without using chemical reagents or any purification procedures, to obtain a high grade PCR-product. A specific DNA fragment of the 18S region of Dekkera bruxellensis and Saccharomyces cerevisia...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
Instituto de Tecnologia do Paraná (Tecpar)
2012-04-01
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Series: | Brazilian Archives of Biology and Technology |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200020 |