Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification

The purpose of this work was to study a rapid yeast DNA extraction by boiling and freeze-thawing processes without using chemical reagents or any purification procedures, to obtain a high grade PCR-product. A specific DNA fragment of the 18S region of Dekkera bruxellensis and Saccharomyces cerevisia...

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Bibliographic Details
Main Authors: Gildo Almeida da Silva, Taís Letícia Bernardi, Patrícia Dayane Carvalho Schaker, Morgana Menegotto, Patricia Valente
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2012-04-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200020