One for all--a highly efficient and versatile method for fluorescent immunostaining in fish embryos.

BACKGROUND: For the detection and sub-cellular (co)-localization of proteins in the context of the tissue or organism immunostaining in whole mount preparations or on sections is still the best approach. So far, each antibody required its own fixation and antigen retrieval protocol so that optimizin...

Full description

Bibliographic Details
Main Authors: Daigo Inoue, Joachim Wittbrodt
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3094454?pdf=render