Gamma-linolenic acid ameliorates Aβ-induced neuroinflammation through NF-κB and MAPK signalling pathways

• Main Authors: Kumju Youn, Seonah Lee, Mira Jun
• Format: Article
• Language: English
• Published: Elsevier 2018-03-01
• Series: Journal of Functional Foods
• Subjects: Alzheimer’s disease; Amyloid β; Gamma-linolenic acid; Inflammation; NF-κB
• Online Access: http://www.sciencedirect.com/science/article/pii/S1756464617307995

Beta-amyloid (Aβ) are known to form senile plaques causing neuroinflammation, which was accepted as the major pathological mechanism in Alzheimer’s disease (AD). To elucidate the molecular mechanism of gamma-linolenic acid (GLA) on neuroprotective actions in inflammation, the effect of GLA on Aβ25–35-stimulated in PC12 cells was investigated. Pre-treatment of GLA significantly decreased Aβ25–35-mediated cytotoxicity through the reduction of ROS and downregulation of caspase-3, thereby attenuating apoptotic morphological alteration. GLA inhibited the production of proinflammatory cytokines including TNF-α and PGE2, and further blocked NF-κB subunit p65 activation by suppressing IκB-α degradation. Mechanistic studies revealed that the inhibitory effect of GLA was accompanied by reducing expression of ERK1/2 and JNK activity but not by p38 MAPK. In conclusion, given that GLA prevents the Aβ25–35 damage via NF-κB signaling pathway, all of which may provide an exciting view of the potential application of GLA as a future research for AD.