A one-step cloning method for the construction of somatic cell gene targeting vectors: application to production of human knockout cell lines
<p>Abstract</p> <p>Background</p> <p>Gene targeting is a powerful method that can be used for examining the functions of genes. Traditionally, the construction of knockout (KO) vectors requires an amplification step to obtain two homologous, large fragments of genomic D...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-10-01
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| Series: | BMC Biotechnology |
| Online Access: | http://www.biomedcentral.com/1472-6750/12/71 |