Surgical protocol for permanent ligation of the left anterior descending coronary artery in mice to generate a model of myocardial infarction

Surgical protocol for permanent ligation of the left anterior descending coronary artery in mice to generate a model of myocardial infarction

Summary: Myocardial infarction (MI) is one of the most common causes of death worldwide. Animal models for MI are useful for studying the pathophysiology and developing therapies. Here, we describe a surgical protocol for permanent ligation of the left anterior descending coronary artery in mice, wh...

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Main Authors: Kazuaki Maruyama, Kazuaki Naemura, Kenji Yoshihara, Kyoko Imanaka-Yoshida, Hiroki Kurihara, Sachiko Miyagawa-Tomita
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Health Sciences
Model Organisms
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721004810
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spelling doaj-8deb3570fc86481aaf3a0fddee3f07f52021-09-19T05:01:11ZengElsevierSTAR Protocols2666-16672021-09-0123100775Surgical protocol for permanent ligation of the left anterior descending coronary artery in mice to generate a model of myocardial infarctionKazuaki Maruyama0Kazuaki Naemura1Kenji Yoshihara2Kyoko Imanaka-Yoshida3Hiroki Kurihara4Sachiko Miyagawa-Tomita5Department of Physiological Chemistry and Metabolism, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; Department of Pathology and Matrix Biology, Graduate School of Medicine, Mie University Graduate School of Medicine, 2-174 Edobashi Tsu, Mie 514-8507, Japan; Corresponding authorDepartment of Physiological Chemistry and Metabolism, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, JapanHeart Center, Department of Pediatric Cardiology, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, JapanDepartment of Pathology and Matrix Biology, Graduate School of Medicine, Mie University Graduate School of Medicine, 2-174 Edobashi Tsu, Mie 514-8507, JapanDepartment of Physiological Chemistry and Metabolism, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, JapanDepartment of Physiological Chemistry and Metabolism, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; Heart Center, Department of Pediatric Cardiology, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan; Department of Animal Nursing Science, Yamazaki University of Animal Health Technology, 4-7-2 Minami-Osawa, Hachioji, Tokyo 192-0364, Japan; Corresponding authorSummary: Myocardial infarction (MI) is one of the most common causes of death worldwide. Animal models for MI are useful for studying the pathophysiology and developing therapies. Here, we describe a surgical protocol for permanent ligation of the left anterior descending coronary artery in mice, which mimics human acute coronary syndrome. This protocol includes descriptive step-by-step surgical procedures and high-quality surgical videos, which are useful for performing stable and highly reproducible operations.For complete details on the use and execution of this protocol, please refer to Maruyama et al. (2021)http://www.sciencedirect.com/science/article/pii/S2666166721004810Cell BiologyHealth SciencesModel Organisms
collection DOAJ
language English
format Article
sources DOAJ
author Kazuaki Maruyama
Kazuaki Naemura
Kenji Yoshihara
Kyoko Imanaka-Yoshida
Hiroki Kurihara
Sachiko Miyagawa-Tomita
spellingShingle Kazuaki Maruyama
Kazuaki Naemura
Kenji Yoshihara
Kyoko Imanaka-Yoshida
Hiroki Kurihara
Sachiko Miyagawa-Tomita
Surgical protocol for permanent ligation of the left anterior descending coronary artery in mice to generate a model of myocardial infarction
STAR Protocols
Cell Biology
Health Sciences
Model Organisms
author_facet Kazuaki Maruyama
Kazuaki Naemura
Kenji Yoshihara
Kyoko Imanaka-Yoshida
Hiroki Kurihara
Sachiko Miyagawa-Tomita
author_sort Kazuaki Maruyama
title Surgical protocol for permanent ligation of the left anterior descending coronary artery in mice to generate a model of myocardial infarction
title_short Surgical protocol for permanent ligation of the left anterior descending coronary artery in mice to generate a model of myocardial infarction
title_full Surgical protocol for permanent ligation of the left anterior descending coronary artery in mice to generate a model of myocardial infarction
title_fullStr Surgical protocol for permanent ligation of the left anterior descending coronary artery in mice to generate a model of myocardial infarction
title_full_unstemmed Surgical protocol for permanent ligation of the left anterior descending coronary artery in mice to generate a model of myocardial infarction
title_sort surgical protocol for permanent ligation of the left anterior descending coronary artery in mice to generate a model of myocardial infarction
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2021-09-01
description Summary: Myocardial infarction (MI) is one of the most common causes of death worldwide. Animal models for MI are useful for studying the pathophysiology and developing therapies. Here, we describe a surgical protocol for permanent ligation of the left anterior descending coronary artery in mice, which mimics human acute coronary syndrome. This protocol includes descriptive step-by-step surgical procedures and high-quality surgical videos, which are useful for performing stable and highly reproducible operations.For complete details on the use and execution of this protocol, please refer to Maruyama et al. (2021)
topic Cell Biology
Health Sciences
Model Organisms
url http://www.sciencedirect.com/science/article/pii/S2666166721004810
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