Recovery of ascorbic oxidoreductase from crude extract with an aqueous two-phase system in a perforated rotating disc contactor

Recovery of ascorbic oxidoreductase from crude extract with an aqueous two-phase system in a perforated rotating disc contactor

A continuous perforated rotating disc contactor was used to extract the enzyme ascorbic oxidoreductase (E.C.1.10.3.3) from crude extract of Curcubita maxima with an aqueous two-phase system of poly (ethylene glycol) and phosphate salts. The effect of dispersed phase velocity on either protein mass t...

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Bibliographic Details
Main Authors: Ana Lúcia Figueiredo Porto, Leonie Asfora Sarubbo, Keila Aparecida Moreira, Homero José Farias de Melo, José Luís Lima-Filho, Galba Maria Campos-Takaki, Elias Basile Tambourgi
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2004-09-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Efficiency
mass transfer coefficient
Perforated Rotating Disc contactor
aqueous two-phase system
ascorbic oxidoreductase
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500019

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500019

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