Use of isotopically labeled substrates reveals kinetic differences between human and bacterial serine palmitoyltransferase

Use of isotopically labeled substrates reveals kinetic differences between human and bacterial serine palmitoyltransferase

Isotope labels are frequently used tools to track metabolites through complex biochemical pathways and to discern the mechanisms of enzyme-catalyzed reactions. Isotopically labeled l-serine is often used to monitor the activity of the first enzyme in sphingolipid biosynthesis, serine palmitoyltransf...

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Bibliographic Details
Main Authors: Peter J. Harrison, Kenneth Gable, Niranjanakumari Somashekarappa, Van Kelly, David J. Clarke, James H. Naismith, Teresa M. Dunn, Dominic J. Campopiano
Format: Article
Language:English
Published: Elsevier 2019-05-01
Series:Journal of Lipid Research
Subjects:
sphingolipid
biosynthesis
mechanism
regulation
membrane protein
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520322653

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http://www.sciencedirect.com/science/article/pii/S0022227520322653

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